BigFISH smFISH Analysis

A napari-plugin providing an alternative GUI for Big-FISH. Big-FISH is a python package for the analysis of smFISH images.

The plugin provides a graphical user interface for some of the functionality in Big-FISH. Currently implemented are:

• Gaussian-background subtraction
• FISH-spot detection with
  • Elimination of duplicates
  • Auto-detection of threshold
• Dense-region decomposition

The plugin further implements by itself:

• Counting of spots per cell, inside and outside of the nucleus
• Batch processing on a list of images

You can find the user and the api-documentation of napari-bigfish here.

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This napari plugin was generated with Cookiecutter using @napari's cookiecutter-napari-plugin template.

Installation¶

You can install napari-bigfish via pip:

pip install napari-bigfish

Contributing¶

Contributions are very welcome. Tests can be run with tox, please ensure the coverage at least stays the same before you submit a pull request.

License¶

Distributed under the terms of the MIT license, "napari-bigfish" is free and open source software

Issues¶

If you encounter any problems, please [file an issue] along with a detailed description.